analysis of her2 gene amplification using differential pcr in breast cancer patients of isfahan province

Authors

zohreh hojati genetics division, biology department, faculty of sciences, university of isfahan, isfahan, iran

zeinab hallajian genetics division, biology department, faculty of sciences, university of isfahan, isfahan, iran

abolghasem esmaeili genetics division, biology department, faculty of sciences, university of isfahan, isfahan, iran

majid motovali-bashi genetics division, biology department, faculty of sciences, university of isfahan, isfahan, iran

abstract

background: amplification of her2 is seen in 20-30% of breast cancer cases. measurement of her2 gene amplification appears to be of vital importance in planning the treatment schedule for patients with breast carcinoma. the aim of our study was to evaluate her2 amplification status in malignant and benign breast tumors by differential pcr (dpcr). materials and methods: the genomic dna was extracted using the phenol/chloroform extraction procedure from 76 different breast tissues. differential pcr was performed using the dna samples isolated from fresh and paraffin- embedded breast cancer tissues. the relative copy number ratio of target gene (her2) to control gene ( inf-γ ) was measured. dpcr products were then separated by electrophoresis using 2% agarose gel. the intensity of her2 and infγ bands were determined for each sample by imagej software. results: according to the ratio between the band intensity of her2 to infγ in tumour and also normal samples, 7% and 26% rates of her2 amplification were observed in benign and malignant samples respectively. the ratio showed a 2-5 fold increase in her2 gene copy number for tissues with her2 amplification whereas, a one-fold increase was found in other samples. conclusion: differential pcr provides a relatively rapid and inexpensive technique to assess the her2 gene amplification, especially alongside immunohistochemistry as a routine assessing method .

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Analysis of HER2 gene amplification using Differential PCR in breast cancer patients of Isfahan Province

Background: Amplification of HER2 is seen in 20-30% of breast cancer cases. Measurement of HER2 gene amplification appears to be of vital importance in planning the treatment schedule for patients with breast carcinoma. The aim of our study was to evaluate HER2 amplification status in malignant and benign breast tumors by differential PCR (dPCR). Materials and Methods: The genomic DNA was ex...

full text

the study of aaag repeat polymorphism in promoter of errg gene and its association with the risk of breast cancer in isfahan region

چکیده: سرطان پستان دومین عامل مرگ مرتبط با سرطان در خانم ها است. از آنجا که سرطان پستان یک تومور وابسته به هورمون است، می تواند توسط وضعیت هورمون های استروئیدی شامل استروژن و پروژسترون تنظیم شود. استروژن نقش مهمی در توسعه و پیشرفت سرطان پستان ایفا می کند و تاثیر خود را روی بیان ژن های هدف از طریق گیرنده های استروژن اعمال می کند. اما گروه دیگری از گیرنده های هسته ای به نام گیرنده های مرتبط به ا...

15 صفحه اول

Predicting Effects of Clinicopathological Variables on Her2 Gene Amplification by Chromogenic in situ Hybridization (CISH) in IHC Her2 (2+) Breast Cancer Patients; A Study from Iran

Background & Objective: The her2 amplification plays an important role in breast cancer management. Therefore, there is a need for using supplementary molecular methods in IHC equivocal cases. Present study has been conducted to determine the effects of clinicopathological variables on her2 gene amplification by chromogenic in situ hybridization (CISH) in IHC ...

full text

Activating HER2 mutations in HER2 gene amplification negative breast cancer.

UNLABELLED Data from 8 breast cancer genome-sequencing projects identified 25 patients with HER2 somatic mutations in cancers lacking HER2 gene amplification. To determine the phenotype of these mutations, we functionally characterized 13 HER2 mutations using in vitro kinase assays, protein structure analysis, cell culture, and xenograft experiments. Seven of these mutations are activating muta...

full text

Identification of Prognostic Genes in Her2-enriched Breast Cancer by Gene Co-Expression Net-work Analysis

Introduction: HER2-enriched subtype of breast cancer has a worse prognosis than luminal subtypes. Recently, the discovery of targeted therapies in other groups of breast cancer has increased patient survival. The aim of this study was to identify genes that affect the overall survival of this group of patients based on a systems biology approach. Methods: Gene expression data and clinical infor...

full text

esp needs analysis of iranian ma students: a case study of university of isfahan

نیاز سنجی دانشجویان کارشناسی ارشد ایرانی در رابطه با زبان تخصصی: مطالعه ای در دانشگاه اصفهان هدف از این مطالعه بررسی نیازهای یادگیری زبان خارجی دانشجویان کارشناسی ارشد دانشجویان دانشگاه اصفهان در رشته های زیست شناسی، روانشناسی، تربیت بدنی، حسابداری و فلسفه ی غرب میباشد. در مجموع 80 دانشجودر پنج رشته ی متفاوت از دانشگاه اصفهان در این پژوهش شرکت داشتند.علاوه بر این هشتاد دانشجو،25 استاد دروس ت...

15 صفحه اول

My Resources

Save resource for easier access later


Journal title:
research in molecular medicine

جلد ۲، شماره ۴، صفحات ۱۲-۱۷

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023